Tinjauan Pewarnaan Hemaktosilin-Eosin dan Periodic Acid-Schiff terhadap Kerusakan Hati Mencit yang Diinduksi Aloksan

The liver is an organ that serves several functions in the body, including glucose metabolism to provide energy other tissues. Hepatocytes are primary functional cells of liver, which also regulate release via transport protein-2. Hepatocyte injury could occur from toxic substances and diseases such as Diabetes Mellitus. Alloxan organic compound commonly used diabetes research a diabetogenic agent. causes through selective inhibition glucose-stimulated insulin secretion induced formation reactive oxygen species (ROS) promotes pancreatic beta cell necrosis. affects liver's histological condition, hepatocellular structure glycogen content. HE PAS for evaluating this condition. However, both should be reviewed evaluate their abilities. Mice were divided into control test groups, each consisting 5 mice. group was intervein-induced with 25, 50, 100mg/KgBB alloxan on second day arrival. mice's livers then taken seventh day; tissue processing carried out get 20 blocks livers. Two 5-μm-thick paraffin-embedded sections stained hematoxylin-eosin, periodic acid Schiff, respectively. Mice's slides examined microscopically degree concentration further evaluation using ImageJ digital imaging application. This study found microscopical ANOVA tests staining methods successfully produced significant differences between various dose alloxan-induced groups